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Image Search Results
Journal: Scientific Reports
Article Title: Genetic background dominates the susceptibility to ventricular arrhythmias in a murine model of β-adrenergic stimulation
doi: 10.1038/s41598-018-20792-5
Figure Lengend Snippet: Heart rate and physical activity under baseline conditions. ( a ) HR and ( b ) physical activity during day- and night-time calculated from 96 hours ECG recordings as shown in Fig. . Activity was derived as a parameter (activity units; A.U.) from the telemetric transponders and corresponds to the physical activity of the animals in the cage. ( c ) HR normalized to activity. For this, the absolute activity units (A.U.) were binned in the following classes: 0 = 0 A.U., 1 = 0–5 A.U., 2 = 5–10 A.U., 3 = 10–15 A.U., 4 = 15–20 A.U., 5 = 20–25 A.U., 6 = 25–30 A.U., 7 = 30–300 A.U. HR values were then averaged according to the corresponding activity. Day: 7AM-7PM, Night: 7PM-7AM. Data is given as median and 5–95 percentile ( a , b ) and as median and interquartile range ( c ). Balb/c n = 6, C57Bl/6 n = 6, BS n = 6, FVB n = 5. *p < 0.05; **p < 0.01; ***p < 0.001 vs. another strain; #p < 0.05 vs. day, ###p < 0.001 vs. day (multiple comparisons were calculated by two-way ANOVA followed by Sidak post-hoc test).
Article Snippet:
Techniques: Activity Assay, Derivative Assay
Journal: Scientific Reports
Article Title: Genetic background dominates the susceptibility to ventricular arrhythmias in a murine model of β-adrenergic stimulation
doi: 10.1038/s41598-018-20792-5
Figure Lengend Snippet: Heart rate under β-adrenergic modulation. ( a ) HRs under treatment with metoprolol during day- and night-time derived from 96 hours ECG recordings shown in Fig. . ( b ) HR reduction (ΔHR) calculated as the difference between mean HRs under baseline conditions and under β-adrenergic blockade. ( c ) Maximal HRs and ( d ) mean HRs over 5 hours following isoproterenol injection. Day: 7AM-7PM, Night: 7PM-7AM. Data is given as median and 5–95 percentile ( a , c , d ) and as median and interquartile range ( b ). Balb/c n = 6, C57Bl/6 n = 6, BS n = 6, FVB n = 5. *p < 0.05; **p < 0.01; ***p < 0.001 against another strain; ###p < 0.001 against day. Multiple comparisons were calculated by ( a ) two-way ANOVA followed by Sidak post-hoc test, and ( b – d ) one-way ANOVA followed by Newman-Keuls post-hoc test.
Article Snippet:
Techniques: Derivative Assay, Injection
Journal: Communications Biology
Article Title: CCDC141 is a Connectin/Titin and Nesprin-1 binding protein that adapts cardiomyocytes to mechanical stress
doi: 10.1038/s42003-025-09093-6
Figure Lengend Snippet: A Masson’s Trichrome staining of wild-type hearts 6 months after severe transverse aortic constriction (sTAC) surgery. B Immunofluorescence microscopy of adult myocardium 6 months after sTAC, stained with CCDC141-N antibody and counterstained with α-actinin and DAPI. Arrows indicate nuclear localization of CCDC141 in cardiomyocyte nuclei. Arrowheads indicate nuclei of non-cardiomyocytes. C Electrocardiogram recording of CCDC141-KO mouse immediately prior to sudden death following sTAC surgery. D Survival analysis of CCDC141-KO mice after moderate TAC (mTAC) surgery. WT, N = 6. KO, N = 7. E Representative echocardiographic images of CCDC141-WT and KO hearts that survived for more than 3 months post-mTAC. F Fractional shortening of hearts that survived beyond 3 months after mTAC or sham surgery. WT-TAC, N = 4. KO-TAC, N = 2. WT-sham, N = 5. KO-sham, N = 5. ** indicates p < 0.01. G Masson’s Trichrome staining of CCDC141-KO hearts 6 months after mTAC surgery. H Electron microscopy of CCDC141-KO hearts 6 months post-mTAC. Arrows indicate presence (WT) and absence (KO) of endoplasmic reticulum. CCDC141 deficiency induces cardiac failure associated with nuclear morphology disruption.
Article Snippet:
Techniques: Staining, Immunofluorescence, Microscopy, Electron Microscopy, Disruption